FIG. 3.
Specificity of bifunctional aptamer-DOX conjugates. TYE665-labeled DOX-conjugated aptamers were incubated with the bEnd.3, MDA-MB-231, or HEK293T cell line and analyzed by flow cytometry. The MFI was plotted against varying concentrations of DOX-loaded bifunctional aptamers (0–400 nM) at a cell density of 5 × 105 cells/mL. Representative binding curves of TEPP-DOX and TENN-DOX with bEnd.3, MDA-MB-231 cells, and HEK293T cells. Data shown are mean ± SEM (n = 3). MFI, mean fluorescence intensity.