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Assessment of DOX, TEPP, and TEPP-DOX cytotoxicity. (A) Following 48 h of treatment with DOX and TEPP-DOX (0–40 μg/mL), cell viability was assessed using MTT assay and the IC50 value for each treatment was calculated as described in the Materials and Methods section. (B) Cell viability for DOX, TEPP, and TEPP-DOX at 10 μg/mL. Data are representative of three independent experiments (n = 3). IC50, half-maximal inhibitory concentration; MTT, thiazolyl blue tetrazolium bromide.
