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. 2020 Apr 3;40(4):BSR20194352. doi: 10.1042/BSR20194352

Figure 2. MiR-3612 is sponged by ZFPM2-AS1 and serves as a tumor suppressor in ESCC.

Figure 2

(A) The subcellular distribution of ZFPM2-AS1 from lncLocator database. (B) FISH assay was conducted to determine ZFPM2-AS1 localization. (C) RIP assay was performed for confirming the involvement of ZFPM2-AS1 in RISC. (D) Predicted miRNAs for ZFPM2-AS1. (E) Luciferase reporter assay was carried out to validate the interaction between ZFPM2-AS1 and predicted miRNAs. (F) MiR-3612 expression in ESCC cells and HET-1A cell. (G) RNA pull down assay was employed for detecting the combination between ZFPM2-AS1 and miR-3612. (H) MiR-3612 expression was detected in cells transfected with miR-3612 mimics. (I) Binding site between ZFPM2-AS1 and miR-3612 was showed. (J) Luciferase activity of ZFPM2-AS1-WT or ZFPM2-AS1-MUT was estimated in ESCC cells with the transfection of miR-3612 mimics or NC mimics. (K) Effect of miR-3612 mimics on cell proliferation. (L) Cell apoptosis was assessed by TUNEL assay after the transfection of miR-3612 mimics. (M and N) Wound healing and transwell assays were utilized for determining the effect of miR-3612 overexpression on cell migration and invasion; **P <0.01.