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. 2020 Apr 3;40(4):BSR20194352. doi: 10.1042/BSR20194352

Figure 3. TRAF4 is a target gene of miR-3612.

Figure 3

(A) Predicted mRNAs were showed by Venn diagram. (B) CBX5, TAOK1 and TRAF4 expressions in ESCC tissues. (C) The expressions of CBX5, TAOK1 and TRAF4 in ESCC cells transfected with miR-3612 mimics. (D) TRAF4 expression in ESCC cells and HET-1A cell. (E) The potential interaction between TRAF4 and miR-3612 was validated by RNA pull-down assay. (F) RIP assay was conducted for measuring the binding between TRAF4 and miR-3612. (G) Predicted binding site of miR-3612 on TRAF4. (H) Luciferase activity of TRAF4-WT or TRAF4-MUT was estimated in ESCC cells with the transfection of miR-3612 mimics or NC mimics. (I) Transfection efficiency of pcDNA-TRAF4 in ESCC cells was detected. (J and K) Cell proliferation and apoptosis were determined by transfecting miR-3612 mimics. (L and M) Function of up-regulated miR-3612 on cell migration and invasion; *P <0.05, **P <0.01.