Changes in beta diversity over the disturbance experiment. Pairwise differences in community structure was quantified using pairwise Bray–Curtis dissimilarity and then ordinated using principal coordinates analysis (PCoA). Time is shown by symbol size, and mesocosm temperature is indicated by heat colours, with the brightest red indicating the warmest time point. Control mesocosms are circles, Disturbance are squares and Disturbance + Immigration are triangles. (a) PCoA of the Total community, assessed using sequencing of the 16S rRNA gene. (b) PCoA of the Active community, including only OTUs that had 16S rRNA : rRNA gene > 1.