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. 2020 Apr;6(2):a004838. doi: 10.1101/mcs.a004838

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© 2020 Barnes et al.; Published by Cold Spring Harbor Laboratory Press

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Figure 2. — Fluorescence in situ hybridization (FISH) panel. The FISH panel results identify the presence of the t(8;22) translocation in both patients. Two hundred cells were analyzed for disruption in FGFR1 using FGFR1 flanking probes, and cases were considered positive if >15% of cells displayed split signals. The Case 1 FISH panels (A) were analyzed using FGFR1 separation probe (Cytocell), and the Case 2 FISH panel (B) was performed using a FGFR1 break-apart probe (Poseidon). Both panels demonstrated der(8) and der(22) along with fusion t(8;22).