Figure 3.

Sanger sequencing. Sanger sequencing identified the same breakpoint in the BCR–FGFR1 fusions found in both patients. cDNA was created from patient RNA samples via reverse transcription polymerase chain reaction (RT-PCR) using a BCR forward and FGFR1 reverse primer. After purification, sequencing was performed with the same primers and compared to BCR–FGFR1 fusions described in a previous report (Landberg et al. 2017). Sanger sequencing trace files for Case 1 (A) and for Case 2 (B) demonstrate the same breakpoint, which matches the previous sequences reported in the literature.