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. 2020 Mar 30;11:397. doi: 10.3389/fimmu.2020.00397

FIGURE 2.

FIGURE 2

Treml4 is expressed in atherogenic oxLDL-loading of BMDM and in diseased aortas of Apoe–/–. (A) Treml4 expression in BMDM after no stimulation (M0) or LPS/IFNγ(M1) for 16 h. **P < 0.01, n = 5 biological replicates (unpaired Student’s t-test). (B,C) Fold change expression of Treml4 and Cd36 in BMDM after overnight incubation with oxLDL (30 μg/ml) in lipoprotein-free media, **p < 0.01, ****p < 0.0001, n = 8 biological replicates (unpaired Student’s t-test). (D) Flow cytometry analyses of Treml4 expression in BMDM after overnight treatment with oxLDL. Representative plot of two independent experiments, n = 3. (E) qPCR analyses of Treml4 and Trem2 expression in thoracic aorta from wt and Apoe–/– mice. *P < 0.05, n = 5 male mice per genotype (unpaired Student’s t-test). (F) Flow cytometry analysis of Treml4 expression in Cd11b+, Ly6Chi, Ly6neg or LyC6int monocyte population in Apoe–/– mice.