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. 2020 Mar 30;11:397. doi: 10.3389/fimmu.2020.00397

FIGURE 5.

FIGURE 5

The effect of Treml4 on lesional macrophages and monocytic populations. (A) Representative images of foam cell/macrophage areas in Movat pentachrome-stained or MOMA-2 immunofluorescence in aortic sinus lesions, scale bar = 100 μm. (B) Histological quantification of foam cell areas within lesions and (C) percentage of MOMA-2 positive area. *P < 0.05, **P < 0.01 (Student’s t-test). (D) Serum Ccl2 levels in 16 week western diet-fed mice. *P < 0.05, ns = not significant, n = 5 per genotype (One-way ANOVA analysis with Tukey’s multiple comparison test). (E) Ccl2 fold change in bone marrow-derived macrophages left untreated or treated with 25 μg oxLDL overnight. (F) Percentage of circulating Ly6Chi and Ly6Clo monocytes in 10 weeks WD mice, n = 5. (G) Caspase 3 activity by flow cytometry in BMM from Apoe–/– and Apoe–/–/Treml4–/– after treatment with 100 ng/μl LPS (M1), 50 ng/μl IL4 (M2) or 25 μg oxLDL overnight, n = 3 per genotype.