FIGURE 6.

Treml4 regulates extracellular matrix deposition. (A) Representative images of Picrosirius red staining in aortic sinuses visualized under light microscopy and quantification of total collagen. (B) Representative images of Picrosirius red visualized under polarized light and the ratio of collagen type III to type I. *P < 0.05, n = 5 males per genotype (Student’s t-test). (C) Representatives images of Movat Pentachrome-stained brachiocephalic artery sections of mice after 16 weeks of western diet feeding and quantification of cartilaginous metaplasia within atherosclerotic lesions. *P < 0.05, n = 4 per genotype (Student’s i-test). (D) Representative images of Mac-3 positive areas within brachiocephalic artery lesions and quantification of percentage per total lesion area. *P < 0.05, n = 4 (Student’s t-test).