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. 2001 Jun 7;18(2):93–103. doi: 10.1016/0147-9571(95)98850-H

Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California

Jarasvech Chinsangaram 1,∗, Caroline E Schore 1, Walter Guterbock 2, Leon D Weaver 2, Bennie I Osburn 1
PMCID: PMC7133876  PMID: 7621673

Abstract

136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.

Keywords: Group A bovine rotavirus, group B bovine rotavirus, rotaviral diarrhea, reverse transcription-polymerase chain reaction (RT-PCR), polyacrylamide gel electrophoresis (PAGE), short electropherotype

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