Table 1.

Overview of the affinity and the biophysical properties of different chimeras. V_{act 1/2} are the midpoint potentials and k the slope factors of the GV curves, respectively. $\Delta {\text{V}}_{1/2}^{3.1}$ are the shifts in the voltage dependence of channel activation compared to WT Kv3.1 ($\Delta {\text{V}}_{1/2}^{3.1}={\text{V}}_{\text{act\hspace{0.17em}}1/2}$ of channel - V_{act 1/2} of Kv3.1). IC₅₀ is the concentration that induces 50% current inhibition and ${\text{IC}}_{50}/{\text{IC}}_{50}^{3.1}$ the fold difference in gambierol affinity of the channel compared to WT Kv3.1.

Channel	IC50 (nM)	Vact 1/2 (mV)	k (mV)	${\text{IC}}_{50}/{\text{IC}}_{50}^{3.1}$	$\Delta {\text{V}}_{1/2}^{3.1}\left(\text{mV}\right)$
Kv3.1	1.2 ± 0.2	23.5 ± 1.0	5.6 ± 0.3	-	-
Kv2.1	>10,000	12.2 ± 1.4	9.5 ± 0.6	>10,000	−11
Kv3.1_EMcoupling_2.1	2.4 ± 0.2	1.1 ±1.5	5.9 ± 0.2	2.0	−23
Kv3.1_S1-S4_2.1	280 ± 40	34.8 ± 3.0	9.1 ± 0.4	233	12
Kv3.1_S1-S3a_2.1	1.9 ± 0.2	44.3 ± 2.4	9.1 ± 0.9	1.6	21
Kv3.1 S3b-S4 2.1	143 ± 28	52.0 ± 0.8	9.4 ± 1.1	119	28
Kv2.1 V404T	504 ± 28	28.7 ± 1.5	16.7 ± 1.5	420	5
Kv2.1 V404T_S3b-S4_3.1	900 ± 41	115.6 ± 9.1	31.5 ± 7.0	750	92