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. 2020 Mar 30;11:459. doi: 10.3389/fimmu.2020.00459

Figure 8.

Figure 8

Overexpression analysis of CiJNK and CiMKK4/CiMKK7 in HEK293T cells. HEK293T cells were transiently co-transfected with pRL-TK (10 ng per well), reporter vector (100 ng per well), and expression vector (250 ng per well). (A) The effects of CiJNK and CiMKK4/CiMKK7 expression on the activity of the AP-1 reporter gene. (B) A direct protein–protein interaction between CiJNK and CiMKK4 or CiMKK7 was found using a mammalian two-hybrid system. Significant differences are indicated by different letters (P < 0.05).