Fig. 4.

Effects of mutations on ATPase and RTPase activities. (A) ATPase. Each reaction contained 30 ng indicated protein and 0.2 mM ATP (plus 1 μCi [γ-³²p]ATP) in a final 3 μl buffer solution. The reaction was performed at 20 °C for 1 min. The reaction products were analyzed by TLC and visualized by autoradiography. (B) RTPase. The conditions were as in part A except that 0.4 μg [γ-³²p]RNA was used as the substrate. The relative activity was estimated according to the pixels of released phosphates by considering WT as 100%.