Fig. 6.

Affinity of variant BaMV helicase-like domains for 3′UTR. (A) Purity of the assayed proteins was examined by SDS-PAGE. (B) UV-induced crosslinking assay. The indicated protein was incubated with a ³²P-labeled probe, which contains the rABC domains of the 3′UTR, for 10 min and irradiated by UV under conditions as described in Materials and methods. After treatment with RNase, the crosslinked proteins were resolved on SDS-PAGE and visualized with a phosphorimager. The relative ability to bind the RNA probe in comparison to WT is indicated after normalization to the loaded protein.