Fig. 9.

Alignment of some proteins belonging to helicase superfamily 1. The helicase motifs are shown in italic with consensus residues marked in bold. BaMV, PVX, FMV, and BMV represent the helicase-like domains from Bamboo mosaic virus, Potato virus X, Foxtail mosaic virus, and Brome mosaic virus, respectively. PcrA is a DNA helicase from Bacillus stearothermophilus. The residues selected for mutagenesis in this study are marked with asterisks. The residues involved in the binding of PcrA to nucleic acids are underlined. BaMV, PVX, and FMV are members of the potexvirus genus, while BMV belongs to bromovirus.