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. 2004 Oct 15;11(10):1445–1453. doi: 10.1016/j.chembiol.2004.08.011

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Copyright © 2004 Elsevier Ltd. All rights reserved.

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Activity of 3CL^pro with the Fluorogenic Peptide Substrate

(A) Michaelis-Menton plot to determine K_m and the observed k_cat.

(B) A plot of observed assay rates containing 100 μM substrate and varying concentrations of 3CL^pro demonstrates assay linearity with respect to proteinase concentration.