Table 1.
Overview of experiments
| Experiment | Genotypes (DAE) | Plants measured | Traits measured (no. of genotypes) |
|---|---|---|---|
| Aus1 | EVA (73–83) | 3 (+N) | Single point, modelled, and leaf structural traits (16) |
| 3 (–N) | |||
| Aus2 | BYPB (48–56) | 2 (+N) | Single point, modelled, and leaf structural traits (30) |
| 2a (–N) | Rubisco in vitro (5) | ||
| Aus3 | BYPB (46–54) | 4a | Single point and modelled traits (25) |
| Leaf structural traits (28) | |||
| Rubisco in vitro (5) | |||
| CA (60–67) | 4a | Single point, modelled, and leaf structural traits (20) | |
| Mex1 | CB (67–82) | 3 | Single point traits (30) |
| SPAD (30) | |||
| CA (88–103) | 3 | Single point and modelled traits (29) | |
| Leaf structural traits (30) | |||
| Cabinet | Merinda (51–53) | 4 | Single point, modelled, and leaf structural traits, and Rubisco in vitro (4) |
| Espada (39 and 50) | 2 | ||
| Mace (57–59) | 4 | ||
| Hawkeye (58–59) | 3 |
Aus1, glasshouse experiment, Australia (2012); Aus2, glasshouse experiment, Australia (2012); Aus3, field experiment GES-CSIRO Australia (2013); Mex1, field experiment, CENEB-CIMMYT, Mexico (2012–2013); Cabinet, glasshouse grown and measured in growth cabinet (2014), Australia; Stage B, before anthesis; Stage A, after anthesis; DAE, days after emergence; –N, low nitrogen treatment; +N, normal nitrogen treatment
a Two plants were measured in the same pot in Aus2 or the same plot in Aus3. Single point traits (A, gs, and Ci/Ca), modelled traits (Vcmax25, J, and J/Vcmax), and leaf structural traits (Nmass, LMA, Narea, and SPAD).