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. 2020 Apr 2;13:2807–2817. doi: 10.2147/OTT.S240803

Figure 3.

Figure 3

Autophagy inhibition suppresses the antitumor activity of IRI in gastric cancer cells. (A) MGC803 and SGC7901 cells were pretreated with 2 mM 3-MA for 24 h or 10 μM CQ for 4 h, and then exposed to 20 μM IRI for 24 h. Western blotting was carried out to analyze LC3, Beclin-1 and P62 protein expression. β-actin was used as the internal control. (B) MTT assay was conducted to analyze cell viability. (C) MGC803 and SGC7901 cells were transfected with Beclin-1 siRNA or siNC, and then exposed to 20 μM IRI for 24 h. Western blotting was carried out to analyze LC3, Beclin-1 and P62 protein expression. β-actin was used as the internal control. (D) Cell viability was determined by the MTT assay. (E, F) Cleaved-PARP and cleaved-caspase-3 protein expression was examined by Western blotting in the indicated cells. β-actin was employed as the internal control. *P < 0.05 compared to Control group; #P < 0.05 compared to IRI group.