Figure 5.

IRI-induced autophagy is related to a ROS-mediated MAPK pathway. (A) MGC803 and SGC7901 cells were pretreated with 5 mM NAC for 1 h and then exposed to 20 μM IRI for 24 h. The expression levels of p-JNK and p-p38 were analyzed by Western blotting. β-actin was used as the internal control. (B) MGC803 and SGC7901 cells were pretreated with 10 µM SP600125 or 10 µM SB203580 for 1 h and then exposed to 20 μM IRI for 24 h. The expression of LC3-I and LC3-II proteins was determined by Western blotting. β-actin served as the internal control.