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. 2020 Apr 2;13:2783–2793. doi: 10.2147/OTT.S232769

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© 2020 Ye et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

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lncRNA SPRY4-IT1 inhibited cell proliferation and promoted apoptosis induced by cisplatin in A549/DDP cells.

Notes: (A) Cell viability was measured by CCK8 assay in A549 and A549/DDP cells with and without transfection of pCDNA-NC or pCDNA-SPRY4-IT1 under different concentrations of cisplatin after treatment. (B) Clonal forming analysis was used to detect the effect of overexpression of SPRY4-IT1 on cell growth under different concentrations of cisplatin at 14 days after treatment. (C) Flow cytometric analysis was used to investigate the effect of SPRY4-IT1 on cell apoptosis induced by cisplatin (cisplatin dose of 0.0, 8.0 μg/mL) at 24 h after treatment. *P <0.05, **P<0.01.

Abbreviations: CCK-8, cell counting kit-8; lncRNA, long noncoding RNA; SPRY4-IT1, SPRY4 intronic transcript 1; RT-qPCR, quantitative real-time polymerase chain reaction.