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. 2020 Apr 2;13:2783–2793. doi: 10.2147/OTT.S232769

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© 2020 Ye et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

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Knockdown of MPZL-1 inhibited cell growth and induced apoptosis of A549/DDP cells.

Notes: (A) MPZL-1 was upregulated in A549/DDP cells by qRT-PCR and Western blot. (B) The expression of MPZL-1 was analyzed by qRT-PCR and Western blot after transfection with si-MPZL-1 in A549/DDP cells at 24 h after treatment. (C) Cell growth was inhibited by downregulating MPZL-1. (D) Clonal forming analysis was used to detect the effect of MPZL-1 low expression on cell growth. (E) Flow cytometric analysis was used to investigate the effect of MPZL-1 low expression on cell apoptosis induced by cisplatin (Cisplatin dose of 0.0, 8.0 μg/mL) at 24 h after treatment. (F) Cell growth was measured by CCK8 assay by transfection with pCDNA-SPRY4-IT1 and pCDNA-MPZL-1 contrast to pCDNA-SPRY4-IT1 overexpression group at 0 h, 24 h, 48 h, and 72 h after treatment. *P <0.05, **P<0.01.

Abbreviations: CCK-8, cell counting kit-8; siRNA, small interfering RNA; RT-qPCR, quantitative real-time polymerase chain reaction; SPRY4-IT1, SPRY4 intronic transcript 1.