Fig 1. Flowchart of all the investigated pre-analytical conditions.

Each condition is shown in a different frame. The number of HVs and patients from which samples were collected and analyzed are displayed on top of each frame. Below the experimental set-up and the used techniques are shown. CP = centrifugation protocol, HVs = healthy volunteers, RT = room temperature, Streck = cfDNA Urine Preserve, UCM = Urine conservation medium.