Fig 3. First-void versus random urine collection.

Analysis was performed using the FragmentAnalyzer on all included samples. Each dot represents the fraction of a urine sample (in mL) of one HV that is needed to detect 50% of the cfDNA present in this urine sample. In urine samples with a small volume (dots on the left of the vertical black line), the first 50 mL of the urine sample suffices to detect 50% of the present cfDNA in the sample (dots below horizontal black line). In the only urine sample with a large volume that was tested (dots on the right of the vertical black line), the first 50 mL of the urine sample does not suffice to detect 50% of the present cfDNA in the sample (dots above horizontal black line). cfDNA = circulating cell-free DNA, gDNA = genomic DNA, HV = healthy volunteer.