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. 2020 Apr 6;15(4):e0231058. doi: 10.1371/journal.pone.0231058

Fig 8. Preparation of cell-free urine by centrifugation.

Fig 8

Five different centrifugation protocols were evaluated using fresh urine samples of HVs. A: Total DNA concentrations analyzed with Qubit fluorometer and ddPCR. B: Amount of cfDNA and gDNA determined with the FragmentAnalyzer. cfDNA = circulating cell-free DNA, CP = centrifugation protocol, CP 1 = 750g 10min + 2,680g 10min, CP 2 = 750g 10min + 3,350g 15min, CP 3 = 750g 20min + 2,680g 10min, CP 4 = 750g 20min, CP 5 = 500g 10min, ddPCR: digital droplet PCR, gDNA = genomic DNA, HV = healthy volunteer.