Table 2.
Second-generation and molecular influenza and respiratory syncytial virus testing, US Food and Drug Administration approved
| Assay | Targets Detected | Methodology | Turnaround Time, min | CLIA Waived | Pediatric References |
|---|---|---|---|---|---|
| BD Veritor System Flu A + B | Influenza A & B | Immunochromatographic assay | 5–10 | Yes | Dunn et al,11 2014 |
| Quidel Sofia Influenza A + B FIA | Influenza A & B | Immunofluorescence-based lateral flow with reader | 3–15 | Yes | Dunn et al,11 2014 |
| Alere i Influenza A & B 2 | Influenza A & B | Isothermal nucleic acid amplification | <15 | Yes | Bell et al,12 2014; Nolte et al,13 2016 |
| Roche cobas Liat Influenza A/B and RSV | Influenza A & B and RSV | Real-time PCR | 20 | Yes | Nolte et al,13 2016 |
| Cepheid Xpert Xpress Flu/RSV Assay | Influenza A & B and RSV | Real-time RT-PCR | 30 | No | — |
| DiaSorin Simplexa Flu A/B & RSV Direct | Influenza A & B and RSV | Real-time RT-PCR | 60 | No | — |
| Quidel Solana Influenza A + B Assay | Influenza A & B | RT-PCR followed by isothermal helicase-dependent amplification | 45 | No | — |