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. 2020 Mar 19;16(3):e1008638. doi: 10.1371/journal.pgen.1008638

Fig 6. Induction of autophagy is not sufficient to suppress spg-7(ad2249)-induced UPRmt.

Fig 6

(A) Plgg-1gfp::lgg-1 expression of spg-7(ad2249) L4 larvae in hypodermal seam cells and intestinal cells upon control(RNAi), vps-4(RNAi) or vps-20(RNAi). Representative images of >80 animals from three independent biological replicates are shown. Scale bar hypodermal seam cells: 5 μm. Scale bar intestinal cells: 20 μm. (B) Western blot analysis of cleaved GFP levels in spg-7(ad2249) upon control(RNAi), vps-4(RNAi) or vps-20(RNAi) using anti-GFP antibodies. Quantification of three independent experiments is shown. Values indicate means ± SD. (C) Fluorescence images of L4 larvae expressing Phsp-6gfp (bcSi9) in wild type (+/+) or spg-7(ad2249). L4 larvae were subjected to control(RNAi), atfs-1(RNAi) or let-363(RNAi) and the F1 generation was imaged. Scale bar: 200 μm. (D) Quantifications of fluorescence images from panel C. After subtracting the mean fluorescence intensity of wild type (+/+) on control(RNAi), the values were normalized to spg-7(ad2249) on control(RNAi). Each dot represents the quantification of fluorescence intensity of 15–20 L4 larvae. Values indicate means ± SD of 3 independent experiments in duplicates. ns: not significant, **P<0.01 using Kruskal-Wallis test with Dunn’s multiple comparison test to control(RNAi).