Skip to main content
. Author manuscript; available in PMC: 2020 Aug 17.
Published in final edited form as: Nat Genet. 2020 Feb 17;52(3):247–253. doi: 10.1038/s41588-020-0579-4

Extended Data Fig. 9. Genomic location of nearest gRNAs to tested causal SNPs and representative flow cytometry plot of CRISPR-Cas9 edited HH cells.

Extended Data Fig. 9

(a) Location of SNPs (red) is shown in reference to the nearest exon (blue) both upstream and downstream of HLA-DQB1. The nearest gRNA sequences used for targeting the regions are highlighted with their corresponding colors (rs71542466 - dark green, rs71542467 - light purple, rs71542468 - purple, rs72844401 - beige/orange, rs4279477 - blue, rs28451423 - light green). Alignments were plotted using SnapGene(v3.2.1). (b) Representative staining of HLA-DQ on CRISPR-Cas9 modified HH cells. Cells were modified with proximal gRNA as shown in (a) and labelled accordingly. Cells stained 7–10 days after modification with HLA-DQ antibodies as a bulk population.