Skip to main content
. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Feb 27;40(4):901–913. doi: 10.1161/ATVBAHA.120.313990

Figure 3. CM reduces blood loss following tail-cut-induced bleeding in hemophilia A mouse model.

Figure 3.

Wild type C57BL/6J mice were injected with 0.25 mg/kg of anti-FVIII (FVIII) antibody (acquired hemophilia A model) (36 female/28 male) or vehicle (saline) (20 female/14 male) at 2 hours prior to tail cut. Then, mice were injected with vehicle or 5.4 mg/kg CM. The distal portion of the tail was surgically removed via a scalpel blade at 1.5-mm tail diameter to induce a moderate bleeding effect. Tails were immersed in 50 mL of saline, pH 7.4 at 37°C. Total blood loss during 10 min was measured as the blood volume collected per mouse weight (μL/g). Bars indicated median with interquartile range, and p values were calculated by Mann-Whitney test.