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. Author manuscript; available in PMC: 2020 Apr 6.
Published in final edited form as: Metallomics. 2020 Mar 25;12(3):346–362. doi: 10.1039/c9mt00301k

Figure 5. Hydrocortisone increases the steady-state level of ZIP14, MT1 and MT2 mRNAs.

Figure 5.

(A) RT-qPCR of CSN2, ZIP14, MT1 and MT2 normalized to either ACTB or RSP9 reference genes. ** p < 0.0002, *** p < 0.0001, unpaired student’s t-test (n=4). (B) RT-qPCR of MT2 normalized to either ACTB or RSP9 in resting media or differentiation media in the presence or absence of different concentrations of the glucocorticoid receptor antagonist RU486 (RU). (C-D) RT-qPCR of ZIP14 (C) and MT1 (D) normalized to either ACTB or RSP9 in resting media or differentiation media with 100 nM RU or vehicle (V) control. * p < 0.01, unpaired student’s t-test (n=4). For MT1, RU treatment did not significantly affect the mRNA level. Each dot represents a biological replicate. Error bars represent standard deviation.