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2-Bromopalmitate decreases ASBT acylation. A, schematic of metabolic labeling by alk-PA in 2BT cells. Alk-PA incorporates into acylated proteins and is conjugated to biotin by click chemistry. Biotinylated proteins are precipitated with NeutrAvidin agarose resin. B and C, incorporation of alk-PA in ASBT in the presence or absence of 25 μm 2-BP. n = 3, *, p < 0.05.
