Figure 4.

Incubation with unsaturated fatty acids decreases ASBT function. A, metabolic labeling of ASBT by alk-OA in 2BT cells (representative Western blotting of three separate experiments). B, [³H]TC uptake in 2BT cells treated with either DMSO, 100 μm PA, or 100 μm OA. n = 6; ****, p < 0.0001 compared with DMSO; ††††, p < 0.0001 compared with PA. C, real-time uptake and area under the curve calculation of bioluminescent bile acid tracer CA-SS-Luc in 2BT cells treated for 15 h with DMSO, 100 μm PA, or 100 μm OA. n = 4; ****, p < 0.0001 compared with DMSO; ††††, p < 0.0001 compared with PA.