Figure 5.
Cys-314 is important for ASBT S-acylation and transport function. A and B, cellular expression (A) and [3H]TC uptake (B) in HEK293 cells transfected with WT or C314S ASBT-V5. n = 3; ****, p < 0.0001 compared with WT. C, Western blotting of bound (S-acylated) and unbound (nonacylated) acyl-RAC fractions from HEK293 cells transfected with WT or C314S ASBT-V5 (representative of three Western blots with similar results). D, a representative Western blotting for cell surface biotinylation of WT and C314S ASBT-V5 fusion proteins.