Figure 5.

The inhibition of necroptosis by Zfp-36 depends on JNK signaling. A–C, WT and Zfp36−/− macrophages were stimulated with LPS (0.01 ng/ml) and zVAD-fmk (10 μm). Some cells were also co-treated with inhibitors against p38MAPK (LY2228820, 4 μm) (A–C), or MK2 (PF3644022, 5 μm) (B and C), or JNK1/2 (SP60012, 50 μm) (B and C). Cell death was evaluated at 24 h by alamarBlue assay (A, C) and the expression of TNFα was measured in the supernatants at 6 h post stimulation (B). Representative data of one experiment of three similar experiments are shown. Graphs show the percentage of viable cells ± S.D. relative to controls. Each experiment was repeated three times. ***, p < 0.001.