Figure 2.
Atypical C–D region of PD-L2 forms a latch that enhances PD-1 binding. A, ribbon representation of the IgV domain of PD-L2 with the C–D latch residues represented as sticks. B and C, SPR sensorgrams of the indicated PD-ligand analytes injected over immobilized PD-1. D, representative, normalized binding curves. E, affinity measurements from independent experiments. F, dissociation rates from independent experiments. Dissociation rates exceeding the range of accurate measurement are shown as >0.4 s−1. Unpaired t tests: **, p < 0.01; ****, p < 0.0001; ns, not significant; RU, response units.