Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (C. elegans) | N2 | Caenorhabditis Genetics Center | Wild Type | |
| Strain, strain background (C. elegans) | CF1903 | Caenorhabditis Genetics Center | glp-1(e2144) | outcrossed three times in C Kenyon's lab |
| Strain, strain background (C. elegans) | YY470 | Caenorhabditis Genetics Center | dcr-1(mg375) | an outcrossed version of YY11 dcr-1(m9375) |
| Strain, strain background (C. elegans) | SHK77 | This paper | dcr-1(mg375) glp-1(e2144) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | CF3152 | Cynthia Kenyon lab | rrf-3(pk1426) | outcrossed three times in C Kenyon's lab |
| Strain, strain background (C. elegans) | SHK55 | This paper | rrf-3(pk1426);glp-1(e2144) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK80 | This paper | ergo-1(gg98) | Strain outcrossed two times in S Henis-Korenblit lab. Total eight outcrosses |
| Strain, strain background (C. elegans) | SHK87 | This paper | glp-1(e2144);ergo-1(gg98) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | YY158 | Caenorhabditis Genetics Center | nrde-3(gg66) | |
| Strain, strain background (C. elegans) | SHK328 | This paper | glp-1(e2144); nrde-3(gg66) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK53 | This paper | sid-1(pk3321) | Strain outcrossed four times in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK56 | This paper | glp-1(e2144);sid-1(pk3321) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | OG497 | Caenorhabditis Genetics Center | unc-119(ed3);drSi13[hsf-1p::hsf-1::GFP::unc-54utr;Cb-unc-119+] | |
| Strain, strain background (C. elegans) | SHK299 | This paper | unc-119(ed3);drSi13[hsf-1p::hsf-1::GFP::unc-54utr;Cb-unc-119+];glp-1(e2144) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK300 | This paper | unc-119(ed3);drSi13[hsf-1p::hsf-1::GFP::unc-54utr;Cb-unc-119+];dcr-1(mg375) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK301 | This paper | unc-119(ed3);drSi13[hsf-1p::hsf-1::GFP::unc-54utr;Cb-unc-119+];dcr-1(mg375) glp-1(e2144) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | AM140 | Caenorhabditis Genetics Center | rmIs132 [unc-54p::Q35::YFP] | |
| Strain, strain background (C. elegans) | SHK409 | This paper | rmIs132 [unc-54p::Q35::YFP];glp-1(e2144) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK412 | This paper | rmIs132 [unc-54p::Q35::YFP];dcr-1 (mg375) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK410 | This paper | rmIs132 [unc-54p::Q35::YFP];dcr-1 (mg375) glp-1(e2144) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | HE250 | Caenorhabditis Genetics Center | unc-52(e669su250) | |
| Strain, strain background (C. elegans) | SHK574 | This paper | unc-52(e669su250);glp-1(e2144) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK575 | This paper | unc-52(e669su250);dcr-1(mg375) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK576 | This paper | unc-52(e669su250);dcr-1(mg375) glp-1(e2144) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) |
SHK415 | This paper | ptp-5.1(tm6122) | Strain outcrossed three times in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK470 | This paper | glp-1(e2144);ptp-5.1(tm6122) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK469 | This paper | dcr-1(mg375);ptp-5.1(tm6122) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK471 | This paper | dcr-1(mg375) glp-1(e2144);ptp-5.1(tm6122) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK405 | This paper | unc-119(ed3);drSi13[hsf-1p::hsf-1::GFP::unc-54utr;Cb-unc-119+];glp-1(e2144);ptp-5.1(tm6122) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK406 | This paper | unc-119(ed3);drSi13[hsf-1p::hsf-1::GFP::unc-54utr;Cb-unc-119+];dcr-1(mg375);ptp-5.1(tm6122) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK407 | This paper | unc-119(ed3);drSi13[hsf-1p::hsf-1::GFP::unc-54utr;Cb-unc-119+];dcr-1(mg375) glp-1(e2144);ptp-5.1(tm6122) | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK619 | This paper | biuEx63[Pptp-5.1::genomic ptp-5.1::gfp+rol-6] | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK622 | This paper | dcr-1(mg375); biuEx63[Pptp-5.1::genomic ptp-5.1::gfp+rol-6] | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK623 | This paper | glp-1(e2144); biuEx63[Pptp-5.1::genomic ptp-5.1::gfp+rol-6] | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK624 | This paper | glp-1(e2144); biuEx63[Pptp-5.1::genomic ptp-5.1::gfp+rol-6] | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK620 | This paper | dcr-1(mg375) glp-1(e2144); biuEx63[Pptp-5.1::genomic ptp-5.1::gfp+rol-6] | Strain created in S Henis-Korenblit lab |
| Strain, strain background (C. elegans) | SHK621 | This paper | dcr-1(mg375) glp-1(e2144); biuEx63[Pptp-5.1::genomic ptp-5.1::gfp+rol-6] | Strain created in S Henis-Korenblit lab |
| Sequence-based reagent | act-1 FW | This paper | qPCR primers | CCAATCCAAGAGAGGTATCCTTAC |
| Sequence-based reagent | act-1 BW | This paper | qPCR primers | CATTGTAGAAGGTGTGATGCCAG |
| Sequence-based reagent | F44E5.5 FW | This paper | qPCR primers | CAGAATGGAAAGGTTGAGATCCTCGCC |
| Sequence-based reagent | F44E5.5 BW | This paper | qPCR primers | ACTGTATTCTCTGGATTACGAGCTGCTTGA |
| Sequence-based reagent | hsp-16.2 BW | This paper | qPCR primers | CTCTCCATCTGAGTCTTCTGAGATTGTTAACA |
| Sequence-based reagent | hsp-16.2 FW | This paper | qPCR primers | CAATTCTTGTTCTCCTTGGATTGATAGCGT |
| Sequence-based reagent | hsp-12.6 BW | This paper | qPCR primers | GATGGAGTTGTCAATGTCCTCGACGAC |
| Sequence-based reagent | hsp-12.6 FW | This paper | qPCR primers | TTGTGCTCCATATGGATTTCAAGAAGTTCTCC |
| Sequence-based reagent | ptp-5.1 FW | This paper | qPCR primers | AAGGCTCCGTCTCCTGCACT |
| Sequence-based reagent | ptp-5.1 BW | This paper | qPCR primers | TCCAGAGACACTTGTTGCTATCGGAG |
| Sequence-based reagent | bw_kpni_ptp-5.1_cds | This paper | cloning primers | GACAATGGTACCTTTCCAGGTCCCATCATACT |
| Sequence-based reagent | fw_PstI_ptp-5.1_Prom | This paper | cloning primers | ATGCCTGCAGCACC TACATTACGCCTGCGC |
| Antibody | anti-HSF-1, rabbit polyclonal Antibody | Abcam | ABE1044 | WB(1:1,000) |
| Antibody | anti-Tubulin mouse monoclonal ascites fluid B-5-1-2 | SIGMA-ALDRICH | T5168 | WB(1:6000) |
| Antibody | anti-Tubulin, mouse monoclonal | DHSB | AA4.3 | WB(1:2,000), RRID:AB_579793 |
| Commercial kit | RNA spike-in kit | Agilent | 5188–5279 | |
| Commercial kit | miRVana miRNA isolation kit (w/phenol) | Ambion | AM1560 | |
| Commercial assay | C. elegans microarray 4 × 23,000 | Agilent | G2519F-020186 | |
| Chemical compound | TRIzol | Ambion | 15596026 | |
| Chemical compound | Linoleic acid sodium salt | Sigma | L8134 | |
| Chemical compound | Maxima SYBR GREEN | Thermo Scientific | K0221 | |
| Instrument | microarray scanner | Agilent | G2565BA | |
| Instrument | CFX-96 real time system | BioRad | ||
| Software, algorithm | Agilent Feature Extraction software | Agilent | version 9.5.1.1 | Agilent Technologies, RRID:SCR_014963 |
| Software, algorithm | Partek Genomics Suite software | Partek | version 6.6 | RRID:SCR_011860 |
| Software, algorithm | DAVID | RRID:SCR_001881 | ||
| Software, algorithm | STRING | RRID:SCR_005223 | ||
| Software, algorithm | SPSS | SPSS | RRID:SCR_002865 |