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. Author manuscript; available in PMC: 2020 Apr 6.
Published in final edited form as: Behav Brain Res. 2019 Nov 21;380:112378. doi: 10.1016/j.bbr.2019.112378

Fig. 2: Effect of different doses of ketamine on intracellular signaling.

Fig. 2:

Ketamine was i.p. injected into mice, 30 min later the animal was sacrificed and the hippocampus dissected out. (a) Representative Western blots. (b) Densitometric quantitation of blots. mBDNF was significantly increased at 5 mg/kg, while significantly decreased at 20 and 50 mg/kg of ketamine, compared to saline-treated control (one-way ANOVA, F(3, 27) = 10.67, p < 0.0001, n = 7 ~ 8 per group). pS6K/S6K showed a tendency toward an increase as a dose-dependent manner but did not achieve statistical significance compared to saline-treated mice (one-way ANOVA, F(3, 28) = 2.009, p = 0.1355, n = 8 per group). pGSK3β/GSK3β did not show any significant changes at 5, 20 or 50 mg/kg ketamine compared to saline-treated mice (one-way ANOVA, F(3, 28) = 0.5598, p = 0.6430, n = 8 per group). Graphs represent mean ± S.E.M., *, p < 0.05, ###, p < 0.001, * indicates comparison with saline control, # indicates comparison between indicated groups. Ket: ketamine