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. 2020 Mar 5;7(3):e703. doi: 10.1212/NXI.0000000000000703

Figure 1. Summary of mNGS diagnostics: improvement over traditional clinical testing.

Figure 1

(A) Comparison of mNGS and qRT-PCR detection of EV-A71, E-30 and CVB in CSF, NP, and fecal samples. Statistics performed using the Fisher exact test, with orange and blue p-values corresponding with mNGS and qRT-PCR results, respectively. (B) Comparison of detection levels for each different experiment, with rpM representing mNGS and Ct values for qRT-PCR. Triangles denote samples identified by mNGS but not qRT-PCR. Statistics performed using a Mann-Whitney test. (C) Heatmap of each individual subject with each body site represented. Boxes with 2 colors represent codetections of different viral species. (D) Comparison of mNGS detection rates to qRT-PCR. Statistics performed using the McNemar test. CVB = Coxsackievirus B; E-30 = echovirus 30; EV-A71 = enterovirus A71; mNGS = metagenomic next-generation sequencing; NP = nasopharyngeal; qRT-PCR = quantitative reverse transcription polymerase chain reaction.