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Cyclooxygenase 2 inhibition blunts human nucleus pulposus (hNP) interleukin (IL)-6 gene expression at 48 and 72 hours after IL-1 activation. hNP cells were treated as described for Fig. 3. n=4 for IL-6 protein level measurement in culture media and n=8 for IL-6 mRNA expression. mRNA level on the y-axis indicates fold changes relative to control untreated cells. Values are mean±standard error of n=4–8. Sc, Sc-58125. #p<0.05 vs. IL-1 alone.
