Fig. 5 –

Effect of immunosuppression reagents on T-cell basal oxygen consumption rate (OCR). Jurkat cells OCR was measured after 4 h of treatment with mycophenolate mofetil (MMF) and 24 h of treatment with cyclosporine A (CsA) at the concentrations shown. After 24 h, cells were recounted and plated on poly-D lysine–treated Seahorse plates in glucose-free RPMI media with 5 mM galactose. Cells incubated for 4 h were immediately replated. A Seahorse bioanalyzer was then used to measure basal OCR following treatment with (A) CsA and (B) MMF. Comparisons between treatment and vehicle control groups and between each treatment group were performed using a two-tailed unpaired Student’s t-test. *P-values were adjusted for multiple comparisons using the Bonferroni correction with a standard significant P value of 0.05. Significant P-values after correction are marked with an asterisk.