Figure 1.

KIF9 is testis‐enriched and localized to mouse flagella. A, Upper, RT‐PCR of Kif9 using RNAs obtained from various tissues of ICR mice. Actb as control. Lower, immunoblot analysis of KIF9 using proteins obtained from various tissues of ICR mice. ACTB as control. B: brain, Th: thymus, Lu: lung, H: heart, S: spleen, Li: liver, K: kidney, Te: testis, O: ovary, and U: uterus. B, Upper, RT‐PCR of Kif9 using RNAs obtained from various postnatal testes of ICR mice. Actb as control. Lower, immunoblot analysis of KIF9 using proteins obtained from various postnatal testes of ICR mice. ACTB as control. C, Localization of KIF9 in spermatozoa. KIF9 is detected in the flagellum. D, Fractionation of mouse spermatozoa. KIF9 was found in the SDS‐soluble fraction. BASIGIN, acetylated tubulin, and AKAP4 were used as makers for the Triton‐soluble, SDS‐soluble, and SDS‐resistant fractions, respectively