Figure 2.

Generation of Kif9‐mutant mice. A, CRISPR/Cas9 targeting scheme. gRNA was designed within exon 2 that contains the start codon. Cyan characters indicate PAM (protospacer adjacent motif) sequence. B, Wave pattern sequence of Kif9. In mutants, 16 bp nucleotides were deleted. C, Genotyping Kif9^−16/−16 mice by StuI digestion. D, The 16 bp deletion caused a P15L mutation resulting in a premature stop codon introduced three amino acids later. E, Protein expression of KIF9 in testis and cauda epididymal spermatozoa. BASIGIN as a loading control