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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: FASEB J. 2020 Feb 17;34(4):5363–5388. doi: 10.1096/fj.201901719RR

Figure 1. TGFβ upregulates IGF-1 and IGF-1 signaling in murine fibroblasts.

Figure 1.

(A) RT-qPCR of Igf-1 gene expression in AKR-2B murine fibroblasts treated with TGFβ compared to vehicle over 24 hours (n=3). (B) RT-qPCR of Igf-1 gene expression in whole lung tissue from intratracheal Bleomycin (BLM, n=5) treated mice compared to Saline (n=5). (C) Western blot and (D) ELISA analysis of IGF-1 ligand expression/secretion following TGFβ or vehicle treatment in AKR-2B fibroblasts over 24 hours (n=3 for both). (E) IGF-1 receptor (IGF-1R) was immunoprecipitated (IP) from AKR-2B murine fibroblasts at the indicated times following treatment with TGFβ (+) or vehicle (−) and Western blotted (WB) for p-IGF-1R (n=3). Data are presented as means −/+ Standard Error of the Mean (SEM) for the number of biological replicates indicated (n). Statistical significance was determined after computing single factor ANOVA and/or unpaired two-tailed Student’s t-test (p<0.05 (*), p<0.01 (**), p<0.005 (***), p<0.001 (****). Results demonstrate that TGFβ stimulates Igf-1 gene expression, ligand production, and IGF-1R activation in murine fibroblasts.