Table 2.
Summary of mouse and human primers used for qRT-PCR.
| Gene (species) | Primer Sequence (5’-3’) | Amplicon Size (bp) |
|---|---|---|
| Acta2 (m) | (F) AGCCATCTTTCATTGGGATGG | 117 |
| (R) CCCCTGACAGGACGTTGTTA | ||
| ACTA2 (h) | (F) GACAATGGCTCTGGGCTCTGTAA | 147 |
| (R) CTGTGCTTCGTCACCCACGTA | ||
| β2m (m) | (F) CTCGGTGACCCTGGTCTTTC | 164 |
| (R) GGATTTCAATGTGAGGCGGG | ||
| B2M (h) | (F) GTATGCCTGCCGTGTGAAC | 139 |
| (R) AAAGCAAGCAAGCAGAATTTGG | ||
| Col1α1 (m) | (F) CGATGGATTCCCGTTCGAGT | 197 |
| (R) CGATCTCGTTGGATCCCTGG | ||
| COL1A1 (h) | (F) GAGGGCCAAGACGAAGACATC | 140 |
| (R) CAGATCACGTCATCGCACAAC | ||
| Col5α3 (m) | (F) CTGCAGAACCTGTGGACGTA | 174 |
| (R) AATGCCCATCTGGGAAGAGC | ||
| COL5A3 (h) | (F) AATTCAGCTCTTCTCGAGCGG | 78 |
| (R) ACTTTTGGTTCGTCTGGCCAA | ||
| COL7A1 (h) | (F) TTACGCCGCTGACATTGTGTT | 93 |
| (R) ACCAGCCCTTCGAGAAAGC | ||
| Ctgf (m) | (F) CACAGAGTGGAGCGCCTGTTC | 163 |
| (R) GATGCACTTTTTGCCCTTCTTAATG | ||
| Eda-fn (m) | (F) GAAGGTTTGCAACCCACTGT | 217 |
| (R) CATCCTCAGGGCTCGAGTAG | ||
| EDA-FN (h) | (F) AGTAACCAACATTGATCGCCCTAA | 76 |
| (R) TTCCCAAGCAATTTTGATGGA | ||
| Igf-1 (m) | (F) GATACACATCATGTCGTCTTCACA | 258 |
| (R) CAGTACATCTCCAGTCTCCTCAGA | ||
| Igf-1 C1 Promoter (m) | (F) AGAGCCTGCGCAATGGAATA | 100 |
| (R) GGCAGGGATAATGAGGCGAA | ||
| Igf-1 C2 Promoter (m) | (F) TGCCTGGGTGTCCAAATGTA | 146 |
| (R) ATTTTGGTAGGTCCGGGTCG | ||
| IGF-1 (h) | (F) GAGCTGGTGGATGCTCTTCA | 112 |
| (R) CATCCACGATGCCTGTCTGA | ||
| IGF-1 C1 Promoter (h) | (F) AAGGCAAATGTTCCCCCAGC | 142 |
| (R) CATTGCGCAGGCTCTATCTG | ||
| IGF-1 C2 Promoter (h) | (F) TCACAAACCCCACCCACAAA | 93 |
| (R) TTTACAGCAGGTCAGGGTGG | ||
| Ins1 (m) | (F) TCTTCTACACACCCAAGTCCCG | 101 |
| (R) CTCCAACGCCAAGGTCTGAA | ||
| Ins2 (m) | (F) CTTCTTCTACACACCCATGTCCC | 101 |
| (R) CCAAGGTCTGAAGGTCACCTG | ||
| INS (h) | (F) CTCACACCTGGTGGAAGCTC | 212 |
| (R) AGAGGGAGCAGATGCTGGTA | ||
| Pai-1 (m) | (F) TTCCAACCAGCATCCCAGAC | 92 |
| (R) CCATGAGACCTTTGTGGGGT | ||
| PAI-1 (h) | (F) GGCCATTACTACGACATCCTG | 150 |
| (R) GGTCATGTTGCCTTTCCAGT | ||
| Tbp (m) | (F) GAAGTTCCCTATAAGGCTGGAAG | 117 |
| (R) AGGAGAACAATTCTGGGTTTGA | ||
| TBP (h) | (F) GCCCGAAACGCCGAATATAATC | 136 |
| (R) GTCTGGACTGTTCTTCACTCTTGG |
Mouse (m; Mus musculus, taxid:10090) and human (h; Homo sapiens, taxid: 9606) primer sets: gene name, NCBI accession number, forward (F) and reverse (R) primer sequences, and qRT-PCR amplicon length.