Table 3.
Summary of antibodies used for Western blot and immunoprecipitation.
| Antigen | Primary Antibody | IP/WB | Dilution | Source |
|---|---|---|---|---|
| AKT | 9272 | WB | 1:10,000 | Cell Signaling |
| p-AKT | 9271 | WB | 1:1,000 | Cell Signaling |
| COL 5 | sc-20648 | WB | 1:5,000 | Santa Cruz |
| CTGF | sc-14939 | WB | 1:5,000 | Santa Cruz |
| ERK1/2 | sc-94 | WB | 1:5,000 | Santa Cruz |
| p-ERK1/2 | 9106 | WB | 1:2,000 | Cell Signaling |
| GAPDH | MAB347 | WB | 1:20,000 | Millipore-Sigma |
| IGF-1 | ab9572 | WB | 1:5,000 | Abcam |
| IGF-1R | 3027 | IP | 2 μl antibody, 400 ug protein, 50 μl protein G beads |
Cell Signaling |
| WB | 1:2,000 | |||
| p-IGF-1R | 3024 | IP | 10 μl antibody, 800 ug protein, 50 μl protein G beads |
Cell Signaling |
| WB | 1:1,000 | |||
| p-IRS1 | 44816G | WB | 1:5,000 | ThermoFisher Scientific |
| mTOR | sc-8319 | WB | 1:2,000 | Santa Cruz |
| S6K | 9202 | WB | 1:10,000 | Cell Signaling |
| p-S6K | 9205 | WB | 1:1,000 | Cell Signaling |
| SGK1 | ab43606 | WB | 1:10,000 | Abcam |
| p-SGK1 | sc-16745-R | WB | 1:1,000 | Santa Cruz |
| SMAD2 | 5339 | WB | 1:2,000 | Cell Signaling |
| p-SMAD2 | WB | 1:5,000 | Leof Lab | |
| SMAD3 | ab28379 | WB | 1:10,000 | Abcam |
| p-SMAD3 | WB | 1:5,000 | Leof Lab | |
| αSMA | A5228 | WB | 1:5,000 | Millipore-Sigma |
Immunoprecipitation (IP), Western blot (WB), rabbit anti-p-Smad3 antibody and anti-p-Smad2 antibody were previously generated to the peptide COOH-GSPSIRCSpSVpS and COOH-CGSPSVRCSpSMpS, respectively [48].