Skip to main content
. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: FASEB J. 2020 Feb 20;34(4):5299–5316. doi: 10.1096/fj.201902958R

Figure 2: Temporal effects of luteinizing hormone (LH) or forskolin on phosphorylation of Dynamin-related protein-1 (DRP1).

Figure 2:

Small bovine luteal cells were treated with LH (10 ng/mL) or forskolin (10 μM) for 0, 0.5, 1, 4, 6, or 24 h. Protein was extracted and subject to western blotting. Progesterone in the culture medium was measured by ELISA. Panel A: Representative western blot analysis on phosphorylation of DRP1 in small luteal cells. Panel B: Fold increase in progesterone following treatment with LH. *Significantly different from control, P < 0.05. Panel C: Densitometric analyses of phospho-DRP1 (Ser637). Panel D: Densitometric analyses of phospho-DRP1 (Ser616). Panel E: Densitometric analyses of phospho-DRP1 (Ser637). Panel F: Densitometric analyses of phospho-DRP1 (Ser616). Panels C-F: Bars represent mean fold changes (means ± sem, n = 3). Open bars: Control 0-h. Closed bars: LH or forskolin-treated cells. Bars with different lettersabcd differ significantly within treatment (P < 0.05).