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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: FASEB J. 2020 Feb 20;34(4):5299–5316. doi: 10.1096/fj.201902958R

Figure 4: Effects of overexpression of Protein Kinase Inhibitor peptide (PKI) on phosphorylation of Dynamin-related protein-1 (DRP1).

Figure 4:

Bovine small luteal cells were treated with replication-deficient adenoviruses containing the complete sequence of endogenous green fluorescent protein (GFP) (Ad.GFP) or Protein Kinase Inhibitor (PKI) (Ad.PKI) to overexpress GFP or PKI. Following infection, cells were treated with vehicle control, luteinizing hormone (LH; 10 ng/mL), forskolin (10 μM), or phorbol 12-myristate 13-acetate (PMA; 20 nM). Succeeding treatment, protein was extracted and subject to western blotting. Panel A: Representative western blot analysis of the phosphorylation of DRP1. Panel B: Densitometric analyses of phospho-DRP1 (Ser637). Panel C: Densitometric analyses of phospho-DRP1 (Ser616). Bars represent means ± sem, n = 3. *Significant difference between treatments as compared to Control, P < 0.05.