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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: FASEB J. 2020 Feb 20;34(4):5299–5316. doi: 10.1096/fj.201902958R

Figure 7: Effects of knockdown of mitochondrial fission factor (MFF) on progesterone production and DRP1 localization in bovine small luteal cells.

Figure 7:

Mitochondrial fission factor (MFF) mRNA was silenced using siMFF in small bovine luteal cells. Following knockdown, cells were treated without (Control) or with luteinizing hormone (LH; 10 ng/mL) for 4 h. Panel A: Densitometric analyses of MFF protein expression. Bars represent means ± sem, n = 4. Inset representative western blot analysis showing expression of MFF in siMFF knockdown small luteal cells. Panel B: Medium progesterone. Bars represent means ± sem, n = 6. *Significant difference between treatment as compared to control, P < 0.05. **Significant difference within treatment (CTL or LH), P < 0.05. Panel C: Representative micrographs of (left to right) Mitotracker (a, g, m and s), MFF (b, h, n and t), DRP1 (c, i, o and u), merge of Mitotracker, MFF, and DRP1 (d, j, p and v), enlarged inlet of colocalization (e, k, q and w), and alpha-Tubulin (f, l, r and x) and (top to bottom) siCTL (a-f), siCTL + LH (10 ng/mL; g-l), siMFF (m-r) and siMFF + LH (10 ng/mL; s-x), enlarged image (white box corresponding to adjacent image). Panel D: Quantitative analysis of the colocalization of DRP1 with Mitotracker. Panel E: Quantitative analysis of the colocalization of DRP1 with MFF. Bars represent means ± standard error; n = 3. Red dashed line indicated analysis cut-off set at 10% colocalization. **Significant difference between treatment (control vs LH), P < 0.05.