Dynamin-related protein-1 (DRP1) mRNA was silenced using siDNM1L in small bovine luteal cells. Following knockdown, cells were treated without (Control) or with luteinizing hormone (LH; 10 ng/mL) for 4 h. Panel A: Representative western blot analysis showing expression of DRP1 and steroidogenic proteins. Panel B: Densitometric analyses of DRP1 protein expression. Bars represent means ± sem, n = 5. Panel C: Densitometric analyses of STAR protein expression. Bars represent means ± sem, n = 3. Panel D: Medium progesterone. Bars represent means ± sem, n = 3. *Significant difference between treatment as compared to control, P < 0.05. **Significant difference within treatment, P < 0.05.