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. Author manuscript; available in PMC: 2020 Apr 6.
Published in final edited form as: ACS Chem Biol. 2019 Jun 25;14(7):1507–1514. doi: 10.1021/acschembio.9b00247

Figure 3.

Figure 3.

Decoration of colloidal drug aggregates with transferrin enhances their endocytosis. MDA-MB-231-H2N cells were treated with colloids (Table S3) with the addition of 500 nM hydrophobic BODIPY dye. Images: cells were treated with lapatinib and fulvestrant colloids stabilized with either (A) transferrin or (B) poly(D,L-lactide-co-2-methyl-2-carboxytrimethylene carbonate)-graft-poly(ethylene glycol) (PLAC-PEG) for 1 h followed by fresh media for 23 h. The cells were labeled with CellMask Green (1×, 5 min) and imaged under live cell conditions. Transferrin-stabilized colloids were visualized as punctate structures inside the cells whereas PLAC-PEG-stabilized colloids were not observed inside the cells. (C) Cells were treated for 3 h, and fluorescence of the BODIPY dye inside the cells was then analyzed by flow cytometry (n = 3 biological replicates, mean ± SD, two-way ANOVA with Tukey’s posthoc test, ***p < 0.001, ****p < 0.0001 compared to all other groups).